human pancreatic islet microtissues 3d insighttm  (Insphero Inc)

Journal: Molecular Metabolism

Article Title: Protection of pancreatic islets from oxidative cell death by a peripherally-active morphinan with increased drug safety

doi: 10.1016/j.molmet.2023.101775

Figure Lengend Snippet: Kom56 increases glucose-stimulated insulin secretion from mouse and human pancreatic islets and enhances the positive effects of cAMP-increasing compounds, including tirzepatide. (A) Insulin secretion from isolated mouse pancreatic islets under low (2 mM) and high glucose concentration (20 mM), and in the absence or presence of different concentrations of Kom56. n = 5 islet batches each. (B) Insulin secretion from mouse pancreatic islets with and without treatment with Kom56 under different glucose concentrations. n = 5 islet batches each. (C–E) Insulin secretion from mouse islets with and without treatment with exendin-4 (Ex-4), forskolin (FSK) as well as tirzepatide (Tirz), and in combination with Kom56. n = 5 islet batches each. (F) Insulin secretion from 3D InSight™ Human Islet Microtissues. n = 4–5 islet batches each. Donor information: 47-year-old male, BMI: 28.4 kg/m 2 . Statistical analysis: (A–F) two-way ANOVA followed by (A) Dunnett's multiple comparison test, (B,F) Sidak's multiple comparison test, and (C–E) Tukey's multiple comparison test. Data are mean ± SEM.

Article Snippet: 3D InSightTM Human Islet Microtissues were provided by InSphero (MT-04-002-01) and represent an in vitro human islet model for diabetes research as well as an alternative to primary human pancreatic islets.

Techniques: Isolation, Concentration Assay, Comparison